Cart 2
  • DAPK Substrate peptide

DAPK Substrate peptide

Not For Human Use, Lab Use Only.

Cat.#: 319824

Size:

* Buy 1 get 1 free (?) What is TFA?

Special Price 110.10 USD

Availability: 1-2 weeks
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    DAPK Substrate peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    H-KKRPQRRYSNVF-OH
  • Sequence
    H-Lys-Lys-Arg-Pro-Gln-Arg-Arg-Tyr-Ser-Asn-Val-Phe-OH
  • Length (aa)
    12
  • Peptide Purity (HPLC)
    95.15%
  • Molecular Formula
    C70H115N25O17
  • Molecular Weight
    1578.81
  • Source
    Synthetic
  • Form
    Powder
  • Description

    The DAPK substrate peptide, KKRPQRRYSNVF, was identified through a combination of structural analysis and positional scanning substrate library synthesis. This peptide serves as an efficient substrate for Death-associated protein kinase (DAPK), a calmodulin-regulated serine/threonine kinase implicated in apoptosis and tumor suppression. The peptide features a core phosphorylation motif, RXXSX, with key residues at positions P-3 (arginine) and P0 (serine). Structural studies of the DAPK catalytic domain revealed unique features, such as a basic loop and specific amino acid interactions, that contribute to substrate recognition. The peptide's Km value of approximately 9 μM makes it suitable for enzymatic assays and further investigations into DAPK's catalytic properties.

  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Velentza AV, Schumacher AM, Weiss C, Egli M, Watterson DM. A protein kinase associated with apoptosis and tumor suppression: structure, activity, and discovery of peptide substrates. J Biol Chem. 2001 Oct 19;276(42):38956-65. doi: 10.1074/jbc.M104273200. Epub 2001 Aug 1. PMID: 11483604.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight

• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"