Crosstide peptide

Crosstide peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRPRTSSFAEG-OH

H-Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly-OH

11

95.92%

C₄₈H₇₇N₁₇O₁₇

1164.22

Synthetic

Powder

Crosstide is a synthetic peptide derived from glycogen synthase kinase-3β (GSK-3). Crosstide functions as substrate for Akt/PKB (protein kinase B) and is also recognized by MAPKAP kinase-1 and p70S6K, Crosstide also displays similar selectivity towards PKBα, PKBβ and PKBγ isoforms. The fluorescent and biotinylated versions of crosstide demonstrate similar enzymic activity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Dai (2002) Human serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK-3β) at serine-9 through direct interaction Biochem. Biophys. Res. Comm. 293 (4) 1191 PMID: 12054501
• Baer et al (2005) Activation of a GST-tagged AKT2/PKBbeta. Biochim. Biophys. Acta 1725(3) 340 PMID: 15890450
• Xu et al (2005) WNK1 Activates SGK1 by a Phosphatidylinositol 3-Kinase-dependent and Non-catalytic Mechanism. J. Biol. Chem. 280 34218 PMID: 16081417

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.