CRGDS peptide

CRGDS peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-CRGDS-OH

H-Cys-Arg-Gly-Asp-Ser-OH

5

97.96%

C₁₈H₃₂N₈O₉S

536.56

Synthetic

Powder

CRGDS forms the cell-binding domain of a glycoprotein, Osteopontin (OPN). Although the native form of OPN is active in cell attachment assays, it has been observed that thrombin cleavage of OPN causes substantial enhancement of its attachment properties. This cleavage occurs within residues of the CRGDS sequence raising the possibility of thrombin-cleavage further activating OPN by allowing greater accessibility of the CRGDS domain to cell surface receptors. CRGDS synthetic peptide also mimics the cellular binding site of many adhesive proteins in the extracellular matrix and causes rounding and detachment of spread cells. It induces the dissociation of alpha-actinin and vinculin from the sites of focal contacts.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Nguyen EH, Zanotelli MR, Schwartz MP, Murphy WL. Differential effects of cell adhesion, modulus and VEGFR-2 inhibition on capillary network formation in synthetic hydrogel arrays. Biomaterials. 2014 Feb;35(7):2149-61. doi:10.1016/j.biomaterials.2013.11.054. Epub 2013 Dec 11. PMID:24332391; PMCID: PMC3970236.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.