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  • Chymotrypsin substrate I peptide

Chymotrypsin substrate I peptide

Not For Human Use, Lab Use Only.

Cat.#: 319657

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* Buy 1 get 1 free (?) What is TFA?

Special Price 399.00 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    Chymotrypsin substrate I peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    Suc-GGF-pNA
  • Sequence
    Suc-Gly-Gly-Phe-pNA
  • Length (aa)
    3
  • Peptide Purity (HPLC)
    95.38%
  • Molecular Formula
    C23H25N5O8
  • Molecular Weight
    499.47
  • CAS No.
    68982-90-1
  • PubChem CID
    40520303
  • Source
    Synthetic
  • Form
    Powder
  • Description
    Chymotrypsin substrate I, colorimetric is a colorimetric substrate for chymotrypsin. Chymotrypsin binds and hydrolyzes chymotrypsin substrate I, colorimetric to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of chymotrypsin activity.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Kasafírek, E., and Bartík, M. N-Acylated phenylalanine p-nitroanilides: New substrates for chymotrypsin. Collect. Czech. Chem. Commun. 45, 442-451 (1980).
    • Pourzand C, Watkin RD, Brown JE, Tyrrell RM. Ultraviolet A radiation induces immediate release of iron in human primary skin fibroblasts: the role of ferritin. Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6751-6. doi: 10.1073/pnas.96.12.6751. PMID: 10359784; PMCID: PMC21987.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"