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  • ChMAP-28

ChMAP-28

Cat.#: 318785

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Optional Service: TFA RemovalWhat's this?

Special Price 212.6 USD

Availability: 3 weeks
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Product Information

  • Product Name
    ChMAP-28
  • Documents
  • Quantity/Unit
    1 Vial
  • Sequence
    H-GRFKRFRKKLKRLWHKVGPFVGPILHY-OH
  • Three letter code
    H-Gly-Arg-Phe-Lys-Arg-Phe-Arg-Lys-Lys-Leu-Lys-Arg-Leu-Trp-His-Lys-Val-Gly-Pro-Phe-Val-Gly-Pro-Ile-Leu-His-Tyr-OH
  • Length (aa)
    27
  • Peptide Purity (HPLC)
    95.2
  • Molecular Formula
    C163H255N49O29
  • Molecular Weight
    3365.07
  • Source
    Synthetic
  • Additional Information
    ChMAP-28 was predicted bioinformatically based on the sequence data of the precursor protein MAP-28 (GenBank AJ243126.1). The ChMAP-28 amino acid sequence was translated from mRNA for the corresponding precursor protein as a 27-residue peptide without the C-terminal glycine, a common amidation signal in cathelicidins. It has molecular mass of 3365 Da and the amino acid sequence GRFKRFRKKLKRLWHKVGPFVGPILHY including eleven basic residues. The goat peptide is highly homologous to the bovine α-helical cathelicidin BMAP-27. ChMAP-28 was found to be toxic toward tumor cells at concentrations of <10 μM, significantly less toxic toward normal cells and low hemolytic. The cytotoxic effect of ChMAP-28 is revealed in 15 min and does not increase during following incubation. Its cell death promoting mechanism was not associated with the caspase-dependent apoptosis, but was rather related to necrosis due to pore formation in the susceptible cell membranes.
  • Storage Guidelines
    Ideally ChMAP-28 should be stored in a freezer at or below -9C. ChMAP-28 should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
  • References
    • Emelianova AA, Kuzmin DV, Panteleev PV, Sorokin M, Buzdin AA, Ovchinnikova TV. Anticancer Activity of the Goat Antimicrobial Peptide ChMAP-28. Front Pharmacol. 2018
  • About TFA salt

    Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

    TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

    TFA Removal Service is recommended for:

    • Peptides that will be used in cellular assays
    • Peptides that will be used as APIs or in manufactured products
    • For hydrophilic peptides containing numerous basic residues

Peptide Property

  • Analysed Sequence:H-GRFKRFRKKLKRLWHKVGPFVGPILHY-OH
  • Chemical Formula:C163H255N49O29
  • Sequence length:27
  • Extinction coefficient:6970 M-1cm-1
  • GRAVY:-0.66
  • Mw average:3365.07
  • Theoretical pI:12.43
  • Data Source:Peptide Property Calculator

GRAVY = grand average of hydropathy

Red: Hydrophobic uncharged residues, like F I L M V W A and P

Blue: Basic residues, like R K H and N-terminal -NH2

Green: Acidic residues, like D E and C-terminal -COOH

Black: Polar uncharged residues, like G S T C N Q and Y

Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"