CF-14 peptide

CF-14 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RIVELTLPRVSVRL-NH2

H-Arg-Ile-Val-Glu-Leu-Thr-Leu-Pro-Arg-Val-Ser-Val-Arg-Leu-NH2

14

95.2%

C₇₄H₁₃₆N₂₄O₁₈

1650.01

Synthetic

Powder

CF-14(RIVELTLPRVSVRL-NH2) is a novel antibacterial peptide, derived from the epidermal mucus of catfish. CF-14 showed antibacterial activity against S. putrefaciens and S. aureus, as well as Escherichia coli compared to negative controls. When the concentration of CF-14 was 62.5 μg/mL, the antibacterial effect of CF-14 against S. putrefaciens was similar to that of 50 μg/mL of ampicillin. CF-14 displays potent activity against gram-negative (S. putrefaciens) bacteria and gram-positive bacteria (S. aureus). Further, the MIC of CF-14 against S. putrefaciens was 62.5 μg/mL. The antimicrobial activity of CF-14 showed no obvious change after heat treatment for 30 min at either at 40 °C, 60 °C, or 80 °C, and slight inactivation after treatment at 100 °C for 30 min. CF-14 remained stable after 24 h after exposure to a pH values ranging from 4.0 to 12.0, but the antimicrobial activity of CF-14 decreased after exposure to an acidic environment (pH < 4). Finally, CF-14 was susceptible to proteases as no antimicrobial activity was observed after treatment with trypsin, pepsin, or proteinase K. CF-14 has the ability to enhance the membrane permeability of S. putrefaciens. Moreover, observations performed using scanning electron and confocal microscopy indicated that CF-14 could penetrate into the cell membranes of S. putrefaciens and accumulate in bacterial cells, but did not break down cell membranes. Further, electrophoresis analysis demonstrated that CF-14 possesses DNA-binding affinity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Li T, Liu Q, Wang D, Li J. Characterization and antimicrobial mechanism of CF-14, a new antimicrobial peptide from the epidermal mucus of catfish. Fish Shellfish Immunol. 2019

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.