CATHPb1 peptide

CATHPb1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KRFKKFFRKIKKGFRKIFKKTKIFIGGTIPI-OH

H-Lys-Arg-Phe-Lys-Lys-Phe-Phe-Arg-Lys-Ile-Lys-Lys-Gly-Phe-Arg-Lys-Ile-Phe-Lys-Lys-Thr-Lys-Ile-Phe-Ile-Gly-Gly-Thr-Ile-Pro-Ile-OH

31

95.2%

C₁₈₇H₃₀₈N₅₀O₃₄

3800.75

Synthetic

Powder

Python Cathelicidin CATHPb1 has protective effect in a broad range of in vivo infection models, especially MRSA and VRSA mice model, by local and systemic administration. CATHPb1 was not only directly antimicrobial and anti-biofilm in vitro, but also immunomodulatory in vivo. The protection was all effective through different administration routes, but was blocked by in vivo depletion of monocyte/macrophages or neutrophils. CATHPb1 can rapidly and massively modulate macrophages/monocytes and neutrophils trafficking to the infection site, and potentiate their bactericidal functions. Meanwhile, CATHPb1 remarkably augmented neutrophil-mediated bacteria killing by facilitating neutrophil extracellular traps (NETs) formation and preventing its degradation. Acting through MAPKs and NF-κB pathways, CATHPb1 selectively enhanced the levels of chemokines while reducing the production of pro-inflammatory cytokines without undesirable toxicities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cai S, Qiao X, Feng L, et al. Python Cathelicidin CATHPb1 Protects against Multidrug-Resistant Staphylococcal Infections by Antimicrobial-Immunomodulatory Duality. J Med Chem. 2018;61(5):2075-2086

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.