Capistruin peptide

Capistruin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GTPGFQTPDARVISRFGFN

Gly-Thr-Pro-Gly-Phe-Gln-Thr-Pro-Asp-Ala-Arg-Val-Ile-Ser-Arg-Phe-Gly-Phe-Asn

19

95.2%

C₉₃H₁₃₇N₂₇O₂₆

2049.24

Synthetic

Powder

Discovered by genome mining based on the known MccJ25-gene coding cluster McjABCD. Capistruin is endoded by the capABCD gene cluster, where capA encodes the precursor polypeptide, capB cleaves the presursor and is likely to promote the cyclization, capC encodes an enzyme that activates the side chain of Asp9 for cyclization, and capD encodes a immunity protein. Showed activity against closely related Burkholderia and Pseudomonas strains. Different from the lasso structure of MccJ25, where the N-terminal Gly forms an amide bond with the carboxyl group of Glu8, capistruin forms a 9-residue macrolactam ring formed between NH of Gly1 and the carboxylic side chain of Asp9. In addition, Arg15 is reponsible for the trapping of the tail in the ring structure. Capistruin has Antimicrobial activity. Capistruin was found in Burkholderia thailandensis E264.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Knappe TA, Linne U, Zirah S, Rebuffat S, Xie X, Marahiel MA. Isolation and structural characterization of capistruin, a lasso peptide predicted from the genome sequence of Burkholderia thailandensis E264. J Am Chem Soc. 2008;130(34):11446-54.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.