CALP1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

CALP1 is an octameric peptide designed to interact with EF-hand motifs, which are calcium-binding domains found in proteins such as calmodulin (CaM). The peptide exhibits a complementary surface contour to an eight-amino-acid stretch within the loop sequence of EF hands, enabling specific binding. CALP1 has been shown to bind the EF hands of CaM, forming a peptide/CaM complex that can modulate calcium-sensitive processes. The peptide is capable of entering cells and influencing calcium-permeable channels, such as glutamate receptor channels and nonselective cation channels, through direct or indirect interactions involving CaM.

CALP1 demonstrates the ability to block calcium influx by targeting the regulatory mechanisms of ion channels rather than their pore regions or ligand-binding sites. Single-channel analysis reveals that CALP1 modulates channel activity by altering open probability and conductance. Its interaction with calcium-sensing domains highlights its role as a novel calcium channel blocker, distinct from traditional inhibitors. The peptide's effects are reversible, indicating a non-accumulative and targeted mechanism of action.

• Manion MK, Su Z, Villain M, Blalock JE. A new type of Ca(2+) channel blocker that targets Ca(2+) sensors and prevents Ca(2+)-mediated apoptosis. FASEB J. 2000 Jul;14(10):1297-306. doi: 10.1096/fj.14.10.1297. PMID: 10877822.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.