Buforin II peptide

Buforin II peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

TRSSRAGLQFPVGRVHRLLRK

H-Thr-Arg-Ser-Ser-Arg-Ala-Gly-Leu-Gln-Phe-Pro-Val-Gly-Arg-Val-His-Arg-Leu-Leu-Arg-Lys-OH

21

97.1%

C₁₀₆H₁₈₄N₄₀O₂₆

2434.8

172998-24-2

Synthetic

Powder

Derived from buforin I after treatment with endoproteinase Lys-C. It displayed a more potent antimicrobial activity than its parent molecule. In TFE, buforin II contains a helix-hinge-helix structure with the first 4 aa disordered. A proline connects helix 1 (residues 5-10) and helix 2 (residues 12-21). MOA: It kills bacteria without lysing the cell. Instead, this peptide translocates aross bacterial membranes effectively. This is a typical peptide example that does not target bacterial membranes but internal targets such as nucleic acids. It has a strong affinity for DNA and RNA in vitro (Park et al 1998. Biochem Biophys Res Commun 244: 253-7). Both buforin II and its derivative buforin IIb (sequence: RAGLQFPVGRLLRRLLRRLLR) showed selective anticancer activity against 62 cancer cell lines (Lee 2008 Cancer Lett 271: 47-51). The sequence of histonin is RAGLQFPVGKLLKKLLKRLKR (updated 12/2008; Jan2013). Buforin II has Antibacterial, Antifungal activity. The source of Buforin II is Bufo bufo gargarizans, Asia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Carvajal SK, Vargas-Casanova Y, Pineda-Castañeda HM, García-Castañeda JE, Rivera-Monroy ZJ, Parra-Giraldo CM. In Vitro Antifungal Activity of Chimeric Peptides Derived from Bovine Lactoferricin and Buforin II against Cryptococcus neoformans var. grubii. Antibiotics (Basel). 2022 Dec 15;11(12):1819.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.