Boc-Glu-Lys-Lys-AMC peptide

Boc-Glu-Lys-Lys-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Boc-EKK-AMC

Boc-Glu-Lys-Lys-AMC

3

95.06%

C₃₂H₄₈N₆O₉

660.75

73554-85-5

53696759

Synthetic

Powder

The peptide Boc-Glu-Lys-Lys-AMC (tert-butoxycarbonyl-glutamic acid-lysine-lysine-amidomethylcoumarin) is a fluorogenic substrate used in assays to measure plasmin generation (PG). Boc-Glu-Lys-Lys-AMC is used to specifically detect plasmin activity and to provide information about the kinetics of plasmin generation during fibrinolysis. However, some assays using this substrate may have limitations, such as not correcting for substrate consumption or inner filter effects, or having issues with substrate specificity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Miszta A, Huskens D, Donkervoort D, Roberts MJM, Wolberg AS, de Laat B. Assessing Plasmin Generation in Health and Disease. Int J Mol Sci. 2021 Mar 9;22(5):2758. doi: 10.3390/ijms22052758. PMID: 33803235; PMCID: PMC7963172.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.