Bim BH3 peptide

Bim BH3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DMRPEIWIAQELRRIGDEFNAYYARR-OH

H-Asp-Met-Arg-Pro-Glu-Ile-Trp-Ile-Ala-Gln-Glu-Leu-Arg-Arg-Ile-Gly-Asp-Glu-Phe-Asn-Ala-Tyr-Tyr-Ala-Arg-Arg-OH

26

95.69%

C₁₄₅H₂₂₂N₄₄O₄₁S

3269.63

Synthetic

Powder

Bim BH3 is a 26-residue fragment from the BH3 only protein Bim. BH3 only proteins are pro-apoptotic members of the B-cell lymphoma 2 (Bcl-2) family of apoptotic regulatory proteins and participate in embryonic development, tissue homeostasis and immunity. The mammalian BH3-only proteins include Bad, Bid, Bik/NBK, Bim/Bod, Bmf, Hrk/DP5, Noxa and Puma/BBC3. Bim BH3 binds to Bcl-2, Bcl-xL and Mcl-1 with very high affinity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zhong et al (2005) Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell 121(7) 1085 PMID: 15989957
• Giam et al (2008) BH3-only proteins and their roles in programmed cell death. Oncogene 27 S128 PMID: 19641498
• Lee et al (2009), High-Resolution Structural Characterization of a Helical α/β-Peptide Foldamer Bound to the Anti-Apoptotic Protein Bcl-xL. Angewandte Chemie International Edition 48 4318 PMID: 19229915

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.