BAR peptide

BAR peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-LEAAPKKVQDLLKKANITVKGAFQLFS-OH

H-Leu-Glu-Ala-Ala-Pro-Lys-Lys-Val-Gln-Asp-Leu-Leu-Lys-Lys-Ala-Asn-Ile-Thr-Val-Lys-Gly-Ala-Phe-Gln-Leu-Phe-Ser-OH

27

95.35%

C₁₃₇H₂₂₉N₃₅O₃₇

2958.48

Synthetic

Powder

BAR peptide is comprised of residues 1167 to 1193 of the SspB (Antigen I/II) protein sequence of S. gordonii (NH2-LEAAPKKVQDLLKKANITVKGAFQLFS-COOH). BAR peptide was developed that potently inhibits P. gingivalis/S. gordonii adherence in vitro and reduces P. gingivalis virulence in a mouse model of periodontitis. While efficacious, one of the challenges to free BAR administration is that it provides relatively transient inhibition of P. gingivalis in the oral cavity. Moreover, to treat established biofilms, relative to initial biofilm formation, higher concentrations of BAR are required.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Daep, C. A., Novak, E. A., Lamont, R. J., and Demuth, D. R. (2011) Structural Dissection and in vivo Effectiveness of a Peptide Inhibitor of Porphyromonas gingivalis Adherence to Streptococcus gordonii. Infect. Immun. 79, 67-74.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.