AS10 peptide

AS10 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KLKKIAQKIKNFFQKLVP-OH

H-Lys-Leu-Lys-Lys-Ile-Ala-Gln-Lys-Ile-Lys-Asn-Phe-Phe-Gln-Lys-Leu-Val-Pro-OH

18

95.47%

C₁₀₅H₁₇₉N₂₇O₂₂

2171.7

Synthetic

Powder

Shorter peptides based on CRAMP, such as AS10 (KLKKIAQKIKNFFQKLVP), were also able to inhibit Candida albicans biofilm growth on Ti disks as well as formation of biofilms from other bacteria. The study also tested the effect of AS10 on human osteoblasts, mesenchymal stromal cells, and endothelial cells in vitro and demonstrated no negative effects on cell viability. Further, AS10 did not interfere with calcium deposition by the osteoblasts or mesenchymal stromal cells or with tube formation by the endothelial cells, which suggests that AS10 could be used as an anti-biofilm coating on implants without affecting cells in the surrounding tissues.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• De Brucker K et al (2014) Derivatives of the mouse cathelicidin-related antimicrobial peptide (CRAMP) inhibit fungal and bacterial biofilm formation. Antimicrob Agents Chemother 58:5395. https://doi.org/10.1128/AAC.03045-14

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.