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Product Name
Anti-Il13 antibody
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Description
Rabbit polyclonal antibody to Il13
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Dog, Pig, Cow, Sheep
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Alternative names
Il- antibody; Il-13 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from mouse il-13 45-56/131(mo)
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Sample:; Lymph node (Mouse) Lysate at 40 ug; Primary: Anti-IL-13 at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 12 kD; Observed band size: 32 kD
Fig2: Sample:; Jurkat(Human) Cell Lysate at 30 ug; Primary: Anti-IL-13 at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 12 kD; Observed band size: 27 kD
Fig3: Sample:; Molt-4(Human) Cell Lysate at 30 ug; Primary: Anti-IL-13 at 1/500 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 12 kD; Observed band size: 32 kD
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-13) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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