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Product Name
Recombinant ALKB5 protein (His tag)
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Description
Recombinant Human RNA Demethylase ALKBH5 (Fragment 74-294) is a high-quality, soluble protein fragment designed for structural and biochemical studies. This fragment, derived from the key catalytic domain of ALKBH5, enables researchers to investigate the enzyme's role in N⁶-methyladenosine (m⁶A) RNA demethylation—a critical post-transcriptional regulatory mechanism in eukaryotes. Ideal for in vitro assays, it supports explorations into mRNA processing, translation, and cellular dynamics such as phase separation.
ALKBH5 is an alpha-ketoglutarate-dependent dioxygenase that specifically demethylates N⁶-methyladenosine (m⁶A) in RNA, the most abundant internal modification in messenger RNA. This enzymatic activity requires molecular oxygen, alpha-ketoglutarate, and iron, and it influences mRNA processing, translation, and nuclear export. ALKBH5 is essential for spermatogenesis by ensuring correct mRNA splicing and 3'-UTR length in male germ cells, and it participates in paraspeckle assembly through liquid
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Protein name
RNA demethylase ALKBH5
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Uniprot ID
Q6P6C2
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Gene Name
ALKBH5; ABH5; OFOXD1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 74-294 aa (Q6P6C2) were fused with N-terminal 6His tag.
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Sequence
MHHHHHHSSG RENLYFQGQQ LQKEEEARKV KSGIRQMRLF SQDECAKIEA RIDEVVSRAE KGLYNEHTVD RAPLRNKYFF GEGYTYGAQL QKRGPGQERL YPPGDVDEIP EWVHQLVIQK LVEHRVIPEG FVNSAVINDY QPGGCIVSHV DPIHIFERPI VSVSFFSDSA LCFGCKFQFK PIRVSEPVLS LPVRRGSVTV LSGYAADEIT HCIRPQDIKE RRAVIILRKT RLDAPRLET
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 27.42 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 29 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant ALKB5 protein SDS-PAGE
M: Marker; Lane 1: ALKB5; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"