ACT1 peptide

ACT1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RQPKIWFPNRRKPWKKRPRPDDLEI-OH

H-Arg-Gln-Pro-Lys-Ile-Trp-Phe-Pro-Asn-Arg-Arg-Lys-Pro-Trp-Lys-Lys-Arg-Pro-Arg-Pro-Asp-Asp-Leu-Glu-Ile-OH

25

99.21%

C₁₅₀H₂₃₈N₄₈O₃₄

3257.81

Synthetic

Powder

ACT1 (α–connexin carboxyl-terminal) is a membrane-permeant peptide incorporating the last nine amino acids of the Cx43 CT, and it is linked to an antennapedia internalization sequence (RQPKIWFPNRRKPWKKRPRPDDLEI). ACT1 is shown to accelerate wound closure, enhance epidermal complexity, reduce granulation tissue area and increase breaking strength and extensibility of skin following cutaneous wounding in mice, which indicates that the Cx43 CT-peptide ACT1 promotes regenerative healing of cutaneous wounds. The peptide may have applications in tissues other than skin, including heart, cornea and spinal cord.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ghatnekar GS, O'Quinn MP, Jourdan LJ, Gurjarpadhye AA, Draughn RL, Gourdie RG. Connexin43 carboxyl-terminal peptides reduce scar progenitor and promote regenerative healing following skin wounding. Regen Med. 2009 Mar;4(2):205-23. doi: 10.2217/17460751.4.2.205. PMID: 19317641; PMCID: PMC2720252.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.