Ac-rER peptide

Ac-rER peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Ac-rER, r=D-Arg

Ac-D-Arg-Glu-Arg-OH

3

95.35%

C₁₉H₃₅N₉O₇

501.53

Synthetic

Powder

Injected intracerebrally around the time of training, RER not only protects against the amnestic effects of down-regulating or functionally blocking APP(amyloid precursor protein), but also against the amnestic effect of the amyloidogenic peptide Aβ1–42. RER binds to proteins at 65–69 kDa, and the binding can be displaced by sAPP fragments. Acetylated-rER (Ac-rER, where the lower case indicates the D-isomeric form of the amino acid), is rapidly transported across the blood-brain-barrier, protects against Aβ-induced memory loss and enhances retention when injected peripherally up to 12 h prior to training.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Mileusnic R, Rose SP. The memory enhancing effect of the APP-derived tripeptide Ac-rER is mediated through CRMP2. J Neurochem. 2011 Aug;118(4):616-25. doi: 10.1111/j.1471-4159.2011.07193.x. Epub 2011 Feb 24. PMID: 21255016.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.