AC-P19M peptide

AC-P19M peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FAKKLAKLKKKLAKLALAL-OH

H-Phe-Ala-Lys-Lys-Leu-Ala-Lys-Leu-Lys-Lys-Lys-Leu-Ala-Lys-Leu-Ala-Leu-Ala-Leu-OH

19

95.39%

C₁₀₂H₁₈₆N₂₆O₂₀

2096.72

Synthetic

Powder

AC-P19M is a novel anticancer peptide designed de novo using in silico methods, as described in the research paper by Shin et al. The peptide was developed through functional prediction and sequence modification based on existing peptide sequences in databases. It exhibits potent anticancer activity against lung cancer cells (A549 and H460) by disrupting cellular membranes, inducing apoptosis, and inhibiting migration and invasion of cancer cells. AC-P19M also demonstrates anti-angiogenic effects by inhibiting VEGF receptor 2 signaling, which is crucial for angiogenesis in endothelial cells. The peptide shows high selectivity for cancer cells with low toxicity towards normal cells, making it a promising candidate for targeted cancer therapy. Physiochemically, AC-P19M has a net charge increase, decreased hydrophobicity, and increased hydrophobic moment compared to its precursor peptide AC-P19. Experimental results confirm that AC-P19M has stronger anticancer activity than AC-P19, with enhanced cytotoxic effects on cancer cells while sparing normal cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Shin MK, Jang BY, Bu KB, Lee SH, Han DH, Oh JW, Sung JS. De Novo Design of AC-P19M, a Novel Anticancer Peptide with Apoptotic Effects on Lung Cancer Cells and Anti-Angiogenic Activity. Int J Mol Sci. 2022 Dec 9;23(24):15594. doi: 10.3390/ijms232415594. PMID: 36555235; PMCID: PMC9779372.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.