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  • Ac-Nle-Pro-Nle-Asp-AMC peptide

Ac-Nle-Pro-Nle-Asp-AMC peptide

Not For Human Use, Lab Use Only.

Cat.#: 319638

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* Buy 1 get 1 free (?) What is TFA?

Special Price 279.00 USD

Availability: 1-2 weeks
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Product Information

  • Product Name
    Ac-Nle-Pro-Nle-Asp-AMC peptide
  • Documents

    Batch to batch variation of the purity

  • Sequence Shortening
    Ac-Nle-Pro-Nle-Asp-AMC
  • Sequence
    Ac-Nle-Pro-Nle-Asp-AMC
  • Length (aa)
    4
  • Peptide Purity (HPLC)
    95.96%
  • Molecular Formula
    C33H45N5O9
  • Molecular Weight
    655.74
  • CAS No.
    355140-49-7
  • PubChem CID
    131698166
  • Source
    Synthetic
  • Form
    Powder
  • Description
    The peptide Ac-Nle-Pro-Nle-Asp-AMC (Acetyl-norleucine-Proline-norleucine-Aspartic acid-amidomethylcoumarin) is a fluorogenic substrate that was designed based on the specificity of the caspase-like sites in proteasomes. Ac-Nle-Pro-Nle-Asp-AMC is cleaved by the caspase-like site, also known as postglutamyl peptide hydrolase (PGPH), with specific activities of 113 and 6.6 nmol/min/mg by the 26S (rabbit muscle) and 20S (yeast) proteasomes, respectively.
  • Storage Guidelines
    Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
  • References
    • Kisselev AF, Garcia-Calvo M, Overkleeft HS, Peterson E, Pennington MW, Ploegh HL, Thornberry NA, Goldberg AL. The caspase-like sites of proteasomes, their substrate specificity, new inhibitors and substrates, and allosteric interactions with the trypsin-like sites. J Biol Chem. 2003 Sep 19;278(38):35869-77. doi: 10.1074/jbc.M303725200. Epub 2003 Jun 18. PMID: 12815064.
  • About TFA salt

    Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

    Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

    Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

    In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.

  • Molar Concentration Calculator

  • Dilution Calculator

  • Percent Concentration Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.

• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.

• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"