A11 peptide

A11 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-YGRKKRRQRRREYVQTVKSSKG-OH

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Glu-Tyr-Val-Gln-Thr-Val-Lys-Ser-Ser-Lys-Gly-OH

22

95.68%

C₁₁₇H₂₀₄N₄₆O₃₂

2767.15

Synthetic

Powder

A11 is a peptide derived from amino acid residues 20-30 of the ANXA1 N-terminus fused to the cell penetrating peptide CPP. A11 blocks ANXA1 competition with the E3 ubiquitin ligase Cbl for binding EphA2, enhancing Cbl-mediated EphA2 ubiquitination and degradation, and reducing the oncogenic mechanism of the ANXA1– EphA2 interaction in nasopharyngeal carcinoma (NPC). A11 can suppress NPC cell growth in vitro and in vivo.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Feng et al (2020) ANXA1 Binds and Stabilizes EphA2 to Promote Nasopharyngeal Carcinoma Growth and Metastasis. Cancer Res. 80(20) 4386 PMID: 32737118

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.