14P2 peptide

14P2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

H-GHPGYINFSYEVLT-OH

H-Gly-His-Pro-Gly-Tyr-Ile-Asn-Phe-Ser-Tyr-Glu-Val-Leu-Thr-OH

14

95.54%

C₇₅H₁₀₅N₁₇O₂₂

1596.73

Synthetic

A 14-residue peptide (14P2, residues Gly8 to Thr21) was identified as a key sequence mediating amelogenin assembly formation, the domain also contains the native single phosphoserine residue (Ser16) of the full-length amelogenin. 14P2 has been recognized for having a strong propensity to self-associate into amyloids.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Zhang J, Bai Y, Wang J, Li B, Habelitz S, Lu JX. Calcium interactions in amelogenin-derived peptide assembly. Front Physiol. 2022 Dec 14;13:1063970. doi: 10.3389/fphys.2022.1063970. Erratum in: Front Physiol. 2023 Apr 04;14:1178589. PMID: 36589425; PMCID: PMC9795176.

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues