α-helical CRF (9-41) peptide

α-helical CRF (9-41) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DLTFHLLREMLEMAKAEQEAEQAALNRLLLEEA-NH2

H-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Met-Leu-Glu-Met-Ala-Lys-Ala-Glu-Gln-Glu-Ala-Glu-Gln-Ala-Ala-Leu-Asn-Arg-Leu-Leu-Leu-Glu-Glu-Ala-NH2

33

95.37%

C₁₆₆H₂₇₄N₄₆O₅₃S₂

3826.34

Synthetic

Powder

α-helical CRF (9-41) is a corticotropin releasing factor receptor (CRF) antagonist with Ki values of 17, 5 and 0.97 nM at human CRF1, rat CRF2α and mouse CRF2β receptors respectively. α-helical CRF (9-41) decreases anxiety like behaviour in the conditioned fear test and reverses the potentiation of startle amplitude produced by administration of CRF in anxiety models. α-helical CRF (9-41) pre-treatment blocks CRF induced food intake inhibition.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Swerdlow et al (1989) Potentiation of acoustic startle by corticotropin-releasing factor (CRF) and by fear are both reversed by α-helical CRF (9-41). Neuropsychopharmacology 2 285 PMID: 2610824
• Skórzewska et al (2009). The effect of CRF and α-helical CRF(9-41) on rat fear responses and amino acids release in the central nucleus of the amygdala. Neuropharmacology 57(2) 148 PMID: 19477189
• Smedh et al (2019) Pretreatment with a CRF antagonist amplifies feeding inhibition induced by fourth ventricular cocaine- and amphetamine-regulated transcript peptide. BMC Neurosci 20 11 PMID: 30885137

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.