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Product Name
Anti-Phospho-FAK (Tyr576/577) Rabbit antibody
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Description
Phospho-FAK (Tyr576/577) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200
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Validations
Western blot detection of FAK(phospho-Tyr576/Tyr577) in Hela cells untreated or UV-treated, using FAK(phospho-Tyr576/Tyr577) Rabbit pAb (dilution 1:1000, upper) or FAK Mouse mAb (200899 dilution 1:500, lower).Predicted band size:125kDa.Observed band size:125kDa.
Western blot analysis of extracts from HT29 cells untreated or treated with PMA using FAK (phospho-Tyr576/Tyr577) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using FAK (phospho-Tyr576/Tyr577) Antibody .
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Background
Swiss-Prot Acc.Q05397.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"