Product Name

FAK antibody

Documents

Description

FAK Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells. Positive IHC detected in human gliomas tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 110 kDa

Tested applications

ELISA, IHC, IP, WB, IF

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

FADK antibody; FADK 1 antibody; FAK antibody; FAK1 antibody; FOCal adhesion kinase 1 antibody; pp125FAK antibody; Protein tyrosine kinase 2 antibody; PTK2 antibody; PTK2 protein tyrosine kinase 2 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of FAK recombinant protein (Accession Number: BC028733). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:1000-1:10000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110455(FAK antibody) at dilution of 1:2000

IP Result of anti-FAK (IP:Catalog No:110455, 3ug; Detection:Catalog No:110455 1:2000) with mouse brain tissue lysate 8000ug.

Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110455(FAK antibody) at dilution of 1:100 (under 10x lens)

Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:110455(FAK Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has 6 isoforms produced by alternative promoter usage and alternative splicing.

References

Lin CL, Zhang ZX, Xu YJ, Ni W, Chen SX. Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis. Chinese medical journal. 118(1):20-6. 2005.
Ding J, Huang S, Wu S. Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA. Nature cell biology. 12(4):390-9. 2010.
Zhang H, Chen X, Liu B, Zhou L. Effects of stable knockdown of Aurora kinase A on proliferation, migration, chromosomal instability, and expression of focal adhesion kinase and matrix metalloproteinase-2 in HEp-2 cells. Molecular and cellular biochemistry. 357(1-2):95-106. 2011.
Zeng F, Luo F, Lv S. A monoclonal antibody targeting neuropilin-1 inhibits adhesion of MCF7 breast cancer cells to fibronectin by suppressing the FAK/p130cas signaling pathway. Anti-cancer drugs. 25(6):663-72. 2014.
Liu C, Liu Y, Xu XX. Potential effect of matrix stiffness on the enrichment of tumor initiating cells under three-dimensional culture conditions. Experimental cell research. 330(1):123-34. 2015.
Wu Q, He J, Mei W, Zhang Z, Wu X, Sun F. Arene ruthenium(ii) complex, a potent inhibitor against proliferation, migration and invasion of breast cancer cells, reduces stress fibers, focal adhesions and invadopodia. Metallomics : integrated biometal science. 6(12):2204-12. 2014.
Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.

FAK antibody

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