Anti-LC3B (7B12) Mouse antibody

Cat.#: 168309

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Product Information

  • Product Name
    Anti-LC3B (7B12) Mouse antibody
  • Documents
  • Description
    LC3B (7B12) Mouse monoclonal antibody
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Rat
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human MAP1LC3B expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    FC: 1/200 - 1/400

    ELISA: 1/10000

  • Validations

    Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).

    Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).

    Western blot analysis of extracts from A549,MCF7,C6 and Hela cell lysates using LC3B mouse mAb (1:500 diluted).Predicted band size:14KDa.Observed band size:14,16KDa.

    Western blot analysis of extracts from A549,MCF7,C6 and Hela cell lysates using LC3B mouse mAb (1:500 diluted).Predicted band size:14KDa.Observed band size:14,16KDa.

  • Background
    Swiss-Prot Acc.Q9GZQ8.The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"