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Product Name
Anti-KDM1/LSD1 (1E5) Mouse antibody
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Description
KDM1/LSD1 (1E5) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Monkey
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Alternative names
Amine oxidase (flavin containing) domain 2;AOF2;BHC110;BRAF35 HDAC complex protein BHC110;BRAF35-HDAC complex protein BHC110;FAD binding protein BRAF35 HDAC complex antibody; 110 kDa subunit;Flavin-containing amine oxidase domain-containing protein 2;KDM 1;KDM1;Kd antibody
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/100
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Validations
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-KDM1/LSD1 mouse mAb (dilution 1:100).
Western blot detection of KDM1/LSD1 in Hela,HCT116 and COS7 cell lysates using KDM1/LSD1 mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.
Immunoprecipitation analysis of Hela cell lysates using KDM1/LSD1 mouse mAb.
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Background
Swiss-Prot Acc.O60341.Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes.
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