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Product Name
KDM1 antibody
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Description
KDM1 Rabbit Polyclonal antibody. Positive WB detected in HT-1080 cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF7 cells, NIH/3T3 cells, PC-3 cells. Positive IP detected in HeLa cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
AOF2 antibody; BHC110 antibody; KDM1 antibody; KDM1A antibody; KIAA0601 antibody; LSD1 antibody; lysine (K) demethylase 1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KDM1 recombinant protein (Accession Number: NM_001009999). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:112023(KDM1 antibody) at dilution of 1:600
IP Result of anti-KDM1 (IP:Catalog No:112023, 4ug; Detection:Catalog No:112023 1:600) with HeLa cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:112023(KDM1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing.
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