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Anti-Aurora A (2E3) Mouse antibody

Cat.#: 168255

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Product Information

  • Product Name
    Anti-Aurora A (2E3) Mouse antibody
  • Documents
  • Description
    Aurora A (2E3) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human, Monkey
  • Alternative names
    AIK;ARK-1;ARK1;AURA;AURKA;Aurora 2;Aurora A;Aurora Family Kinase 1;aurora kinase A;Aurora Related Kinase 1;Aurora-related kinase 1;Aurora/IPL1 Like Kinase; Aurora/IPL1 Related Kinase 1;Aurora/IPL1-related kinase 1;AURORA2;AYK1;BRAK;Breast Tumor Amplified antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human Aurora Kinase A protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascites containing with 0.03% Proclin300 and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500

    ;ICC: 1/50

  • Validations

    Immunofluorescent analysis of Hela cells labeled with Aurora Kinase A(168255,dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045,dilution 1:100) Rabbit pAb (red). DAPI was used to stain nucleus(blue).

    Immunofluorescent analysis of Hela cells labeled with Aurora Kinase A(168255,dilution 1:50) mouse mAb (green) and Histone H3.1 (Phospho-Ser10)(310045,dilution 1:100) Rabbit pAb (red). DAPI was used to stain nucleus(blue).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using Aurora Kinase A mouse mAb (dilution 1:100).

    Immunocytochemistry staining of HeLa cells fixed with -20u2103 Methanol and using Aurora Kinase A mouse mAb (dilution 1:100).

    Western blot detection of Aurora Kinase A in SW480 and COS7 cell lysates and using Aurora Kinase A mouse mAb (1:500 diluted).Predicted band size: 46KDa.Observed band size:46KDa.

    Western blot detection of Aurora Kinase A in SW480 and COS7 cell lysates and using Aurora Kinase A mouse mAb (1:500 diluted).Predicted band size: 46KDa.Observed band size:46KDa.

  • Background
    Swiss-Prot Acc.O14965.Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizating p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"