-
Product Name
Aurora A antibody
- Documents
-
Description
Aurora A Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, SKOV-3 cells, SW 1990 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 46 kDa
-
Tested applications
ELISA, IHC, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
AIK antibody; ARK 1 antibody; ARK1 antibody; AURA antibody; AURKA antibody; Aurora A antibody; aurora kinase A antibody; Aurora related kinase 1 antibody; Aurora/IPL1 related kinase 1 antibody; AURORA2 antibody; Breast tumor amplified kinase antibody; BTAK antibody; hARK1 antibody; STK15 antibody; STK6 antibody; STK7 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Aurora A recombinant protein (Accession Number: NM_001323305). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:10-1:100
IF: 1:20-1:200
-
Validations
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117073(AURKA antibody) at dilution of 1:10 (under 25x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:117073(AURKA Antibody) at dilution of 1:600
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:117073(AURKA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Serine/threonine kinase 6(STK6),also named as STK15 and AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that STK6 may also be implicated in tumor development and progression. STK6 is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).
-
References
- Liu Y, Hu H, Zhang C. Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas. Oncotarget. 6(35):38257-69. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"