Human BLyS/TNFSF13B (Fc Tag) recombinant protein

Human BLyS/TNFSF13B (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

Tumor necrosis factor ligand superfamily member 13B

BLYS; TNFSF20; D8ERTD387E; ZTNF4; TALL-1; MGC124061; CD257; MGC124060; BAFF; DTL; TALL1; THANK; TNFSF13B

Q9Y275

TNFSF13B; BAFF; BLYS; TALL1; TNFSF20; ZTNF4; UNQ401/PRO738

Human Cells

A DNA sequence encoding the soluble form of human BAFF (Q9Y275-1) (Ala 134-Leu 285) was fused with the Fc region of human IgG1 at the N-terminus.

Human

The recombinant human BAFF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 389 amino acids and has a predicted molecular mass of 43.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhBAFF/Fc monomer is approximately 48-55 kDa due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human BAFF at 1 μg/mL (100 μl/well) can bind human TNFRSF17. The EC₅₀ of human TNFRSF17 is 0.07 μg/mL.
2. Measured in a cell proliferation assay using anti-IgM stimulated mouse splenic cells. The ED₅₀ for this effect is typically 50-150 ng/mL.