-
Product Name
TBC1D5 antibody
- Documents
-
Description
TBC1D5 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells. Positive IHC detected in human kidney tissue. Observed molecular weight by Western-blot: 89 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
KIAA0210 antibody; TBC1 domain family member 5 antibody; TBC1 domain family antibody; member 5 antibody; TBC1D5 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of TBC1D5 recombinant protein (Accession Number: XM_047449299). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:115868(TBC1D5 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115868(TBC1D5 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:115868(TBC1D5 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-TBC1D5 (IP:Catalog No:115868, 4ug; Detection:Catalog No:115868 1:300) with A431 cells lysate 2400ug.
-
Background
TBC1D5, a member of TBC (Tre2/Bub2/Cdc16)1 domain family, is a novel retromer-interacting protein. TBC1D5 is a Rab GTPase-activating proteins (GAPs) proteins that negatively regulates VPS35/29/26 recruitment and causes Rab7 to dissociate from the membrane. TBC1D5 could bridge the endosome and autophagosome via its C-terminal LIR motif, and Rab GAPs are implicated in the reprogramming of the endocytic trafficking events under starvation-induced autophagy.
-
References
- Gomez TS, Gorman JA, de Narvajas AA, Koenig AO, Billadeau DD. Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks. Molecular biology of the cell. 23(16):3215-28. 2012.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"