-
Product Name
Synaptophysin antibody
- Documents
-
Description
Synaptophysin Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, mouse brain tissue, mouse cerebellum tissue. Positive IHC detected in human pituitary adenoma tissue, human brain tissue, human pancreas tissue. Positive IF detected in PC-12 cells. Observed molecular weight by Western-blot: 38 kDa
-
Tested applications
ELISA, WB, IHC, IP, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
SYN antibody; synaptophysin antibody; SYP antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Synaptophysin recombinant protein (Accession Number: NM_003179). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:115768(SYP antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human pituitary adenoma tissue slide using Catalog No:115768(Synaptophysin; SYP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pituitary adenoma tissue slide using Catalog No:115768(Synaptophysin; SYP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of PC-12 cells using Catalog No:115768(Synaptophysin; SYP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-Synaptophysin; SYP (IP:Catalog No:115768, 3ug; Detection:Catalog No:115768 1:1000) with mouse brain tissue lysate 4000ug.
-
Background
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)
-
References
- Lan X, Zhang M, Yang W. Effect of treadmill exercise on 5-HT, 5-HT1A receptor and brain derived neurophic factor in rats after permanent middle cerebral artery occlusion. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology. 35(5):761-6. 2014.
- Tian H, Wang L, Cai R, Zheng L, Guo L. Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model. PloS one. 9(12):e116453. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"