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RUNX1 antibody

Cat.#: 114935

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Special Price 239.0 USD

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Product Information

  • Product Name
    RUNX1 antibody
  • Documents
  • Description
    RUNX1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HEK-293 cells, HL-60 cells, Jurkat cells, MCF7 cells, mouse spleen tissue, mouse thymus tissue, Raji cells, SH-SY5Y cells, Sp2/0 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 52-55 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AML1 antibody; AML1 EVI 1 antibody; AMLCR1 antibody; CBF alpha 2 antibody; CBFA2 antibody; EVI 1 antibody; Oncogene AML 1 antibody; PEA2 alpha B antibody; PEBP2 alpha B antibody; PEBP2aB antibody; RUNX1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_001754). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114935(RUNX1 antibody) at dilution of 1:500

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:114935(RUNX1 antibody) at dilution of 1:500

    IP Result of anti-RUNX1 (IP:Catalog No:114935, 4ug; Detection:Catalog No:114935 1:500) with mouse liver tissue lysate 4000ug.

    IP Result of anti-RUNX1 (IP:Catalog No:114935, 4ug; Detection:Catalog No:114935 1:500) with mouse liver tissue lysate 4000ug.

  • Background
    RUNX1, also named as AML1 and CBFA2, acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. RUNX1 inhibits MYST4-dependent transcriptional activation. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"