Product Name

p65; RELA antibody

Documents

Description

p65; RELA Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse lung tissue, NIH/3T3 cells, pig liver tissue, rat lung tissue. Positive IP detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human liver cancer tissue, human stomach tissue. Positive IF detected in HepG2 cells. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 65 kDa

Tested applications

ELISA, IHC, IF, IP, WB, FC

Species reactivity

Human,Mouse,Rat, Pig; other species not tested.

Alternative names

NFKB 3 antibody; NFKB p65 antibody; NFKB3 antibody; p65 antibody; RELA antibody; Transcription factor p65 antibody

Immunogen

Human p65; RELA Recombinant protein (GST tag)

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of p65; RELA recombinant protein (Accession Number: BC011603). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113559(p65 antibody) at dilution of 1:1000

IP Result of anti-p65 (IP:Catalog No:113559, 3ug; Detection:Catalog No:113559 1:1000) with HeLa cells lysate 5000ug.

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113559(p65 antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of HepG2 cells, using RELA antibody Catalog No:113559 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

1X10^6 HeLa cells were stained with 0.2ug p65; RELA antibody (Catalog No:113559, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Background

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

References

Yu N, Di Q, Liu H. Nuclear factor-kappa B activity regulates brain expression of P-glycoprotein in the kainic acid-induced seizure rats. Mediators of inflammation. 2011:670613. 2011.
Gong K, Li W. Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma. Free radical biology & medicine. 51(12):2259-71. 2011.
Tian D, Qiu Y, Zhan Y. Overexpression of steroidogenic acute regulatory protein in rat aortic endothelial cells attenuates palmitic acid-induced inflammation and reduction in nitric oxide bioavailability. Cardiovascular diabetology. 11:144. 2012.
Tang S, Bao H, Zhang Y, Yao J, Yang P, Chen X. 14-3-3ε mediates the cell fate decision-making pathways in response of hepatocellular carcinoma to Bleomycin-induced DNA damage. PloS one. 8(3):e55268. 2013.
Zhang L, Tang H, Kou Y. MG132-mediated inhibition of the ubiquitin-proteasome pathway ameliorates cancer cachexia. Journal of cancer research and clinical oncology. 139(7):1105-15. 2013.
Zhang J, Wang S, Wang K, Zheng C. Herpes simplex virus 1 DNA polymerase processivity factor UL42 inhibits TNF-α-induced NF-κB activation by interacting with p65/RelA and p50/NF-κB1. Medical microbiology and immunology. 202(4):313-25. 2013.
Zhang S, Jiang C, Liu H. Fluoride-elicited developmental testicular toxicity in rats: roles of endoplasmic reticulum stress and inflammatory response. Toxicology and applied pharmacology. 271(2):206-15. 2013.
Luo J, Zhou H, Wang F. The hepatitis B virus X protein downregulates NF-κB signaling pathways through decreasing the Notch signaling pathway in HBx-transformed L02 cells. International journal of oncology. 42(5):1636-43. 2013.

p65; RELA antibody

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