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Product Name
LPP antibody
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Description
LPP Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human small intestine tissue, human kidney tissue. Positive WB detected in mouse colon tissue, HeLa cells, MCF-7 cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 70-75 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Lipoma preferred partner antibody; LPP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LPP recombinant protein (Accession Number: NM_005578). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:112309(LPP Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:112309(LPP Antibody) at dilution of 1:50 (under 10x lens)
1X10^6 HeLa cells were stained with 0.05ug LPP antibody (Catalog No:112309, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.
Immunofluorescent analysis of HeLa cells using Catalog No:112309(LPP Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:112309(LPP Antibody) at dilution of 1:50 (under 40x lens)
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Background
LPP represents LIM domain containing preferred translocation partner in lipoma
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"