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Product Name
Kallikrein 1 antibody
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Description
Kallikrein 1 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in BxPC-3 cells. Positive WB detected in A375 cells, BxPC-3 cells. Observed molecular weight by Western-blot: 45-50kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
hK1 antibody; kallikrein 1 antibody; KLK1 antibody; Klk6 antibody; KLKR antibody; Tissue kallikrein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Kallikrein 1 recombinant protein (Accession Number: NM_002257). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:111901(KLK1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:111901(KLK1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-KLK1 (IP:Catalog No:111901, 4ug; Detection:Catalog No:111901 1:500) with BxPC-3 cells lysate 1520ug.
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Background
KLK1 (tissue kallikrein 1) is a member of the tissue kallikrein family of serine proteases and is the primary kinin-generating enzyme in human airways. It is involved in the control of ionic transport in the renal tubule, an action that may not be kinin-mediated. This protein has 2 isoforms produced by alternative splicing.
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References
- Cerutti JM, Oler G, Delcelo R. PVALB, a new Hürthle adenoma diagnostic marker identified through gene expression. The Journal of clinical endocrinology and metabolism. 96(1):E151-60. 2011.
- Yu HZ, Xie LD, Zhu PL, Xu CS, Wang HJ. Human tissue kallikrein 1 gene delivery inhibits PDGF-BB-induced vascular smooth muscle cells proliferation and upregulates the expressions of p27Kip1 and p2lCip1. Molecular and cellular biochemistry. 360(1-2):363-71. 2012.
- Li Y, Raman I, Du Y. Kallikrein transduced mesenchymal stem cells protect against anti-GBM disease and lupus nephritis by ameliorating inflammation and oxidative stress. PloS one. 8(7):e67790. 2013.
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