Product Name

IDH1 antibody

Documents

Description

IDH1 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human liver cancer tissue. Positive WB detected in HepG2 cells, DU 145 cells, HEK-293 cells, MCF7 cells, MCF-7 cells, mouse liver tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 46kd

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

IDCD antibody; IDH antibody; IDH1 antibody; IDP antibody; IDPC antibody; NADP(+) specific ICDH antibody; Oxalosuccinate decarboxylase antibody; PICD antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of IDH1 recombinant protein (Accession Number: NM_005896). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

Validations

HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111602(IDH1 antibody) at dilution of 1:2000

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:111602(IDH1 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:111602(IDH1 Antibody) at dilution of 1:50 (under 40x lens)

Background

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. Its subunit structure is homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). This antibody is specific to IDH1.

IDH1 antibody

Product Information

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