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Product Name
HEXA antibody
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Description
HEXA Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 54kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
HEXA antibody; Hexosaminidase subunit A antibody; TSD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HEXA recombinant protein (Accession Number: NM_000520). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111302(HEXA antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111302(HEXA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:111302(HEXA antibody) at dilution of 1:50 (under 40x lens)
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Background
Beta-hexosaminidase is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase, The antibody, 11317-1-AP, can recognize both alpha and beta subunit of beta-hexosaminidase. The mature alpha subunit is composed of a major 54kd polypeptide and a minor 6kd polypeptide. The mature beta-subunit originates from its precursor by two proteolytic cleavage events forming 3 chains: 11kd, 24kd and 28kd.
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References
- Pushparaj PN, Tay HK, Wang CC, Hong W, Melendez AJ. VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation. Journal of immunology (Baltimore, Md. : 1950). 183(2):1413-8. 2009.
- Trost M, English L, Lemieux S, Courcelles M, Desjardins M, Thibault P. The phagosomal proteome in interferon-gamma-activated macrophages. Immunity. 30(1):143-54. 2009.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"