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Product Name
Cytokeratin 17 antibody
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Description
Cytokeratin 17 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human cervical cancer tissue. Positive WB detected in HeLa cells, MCF-7 cells. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
39.1 antibody; CK 17 antibody; Cytokeratin 17 antibody; K17 antibody; keratin 17 antibody; KRT17 antibody; PC2 antibody; PCHC1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cytokeratin 17 recombinant protein (Accession Number: NM_000422). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:109797(KRT17 antibody) at dilution of 1:2000
Immunofluorescent analysis of HeLa cells using Catalog No:109797(KRT17 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109797(KRT17 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:109797(KRT17 Antibody) at dilution of 1:50 (under 40x lens)
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.
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