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Product Name
CISD2 antibody
- Documents
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Description
CISD2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human pancreas tissue, human spleen tissue, human testis tissue. Positive FC detected in HepG2 cells. Positive IP detected in mouse brain tissue. Positive WB detected in mouse kidney tissue, HeLa cells, human brain tissue, mouse brain tissue, mouse heart tissue. Observed molecular weight by Western-blot: 13-15 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDGSH iron sulfur domain 2 antibody; CDGSH2 antibody; CISD2 antibody; ERIS antibody; Miner1 antibody; MitoNEET related 1 protein antibody; NAF 1 antibody; WFS2 antibody; ZCD2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CISD2 recombinant protein (Accession Number: NM_001008388). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:109322(CISD2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:109322(CISD2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CISD2 (IP:Catalog No:109322, 3ug; Detection:Catalog No:109322 1:700) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:109322(CISD2 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
1X10^6 HepG2 cells were stained with 0.2ug CISD2 antibody (Catalog No:109322, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.
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References
- Lu S, Kanekura K, Hara T. A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome. Proceedings of the National Academy of Sciences of the United States of America. 111(49):E5292-301. 2014.
- Wang L, Ouyang F, Liu X. Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway. Oncotarget. 2015.
- Chen B, Shen S, Wu J. CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma. International journal of clinical and experimental pathology. 8(10):13725-38. 2015.
- Bai XC, Rajendra E, Yang G, Shi Y, Scheres SH. Sampling the conformational space of the catalytic subunit of human γ-secretase. eLife. 4:. 2015.
- Jones MA, Amr S, Ferebee A. Genetic studies in Drosophila and humans support a model for the concerted function of CISD2, PPT1 and CLN3 in disease. Biology open. 3(5):342-52. 2014.
- Liu L, Xia M, Wang J, Zhang W, Zhang Y, He M. CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer. Medical oncology (Northwood, London, England). 31(9):183. 2014.
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