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Product Name
ASF1A antibody
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Description
ASF1A Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive WB detected in NIH/3T3 cells, HeLa cells. Observed molecular weight by Western-blot: 20-23 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ASF1A antibody; CCG1 interacting factor A antibody; CGI 98 antibody; CIA antibody; DKFZp547E2110 antibody; hAsf1 antibody; hAsf1a antibody; hCIA antibody; Histone chaperone ASF1A antibody; HSPC146 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASF1A recombinant protein (Accession Number: NM_014034). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:108223(ASF1A antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:108223(ASF1A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.
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