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Product Name
ASF1A antibody
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Description
ASF1A Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 23kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ASF1A antibody; CCG1 interacting factor A antibody; CGI 98 antibody; CIA antibody; DKFZp547E2110 antibody; hAsf1 antibody; hAsf1a antibody; hCIA antibody; Histone chaperone ASF1A antibody; HSPC146 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ASF1A recombinant protein (Accession Number: NM_014034). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108222(ASF1A antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:108222(ASF1A antibody) at dilution of 1:10 (under 10x lens)
IP Result of anti-ASF1A (IP:Catalog No:108222, 4ug; Detection:Catalog No:108222 1:800) with HEK-293 cells lysate 2800ug.
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Background
Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.
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References
- Ambagala AP, Bosma T, Ali MA. Varicella-zoster virus immediate-early 63 protein interacts with human antisilencing function 1 protein and alters its ability to bind histones h3.1 and h3.3. Journal of virology. 83(1):200-9. 2009.
- Jiang WQ, Nguyen A, Cao Y, Chang AC, Reddel RR. HP1-mediated formation of alternative lengthening of telomeres-associated PML bodies requires HIRA but not ASF1a. PloS one. 6(2):e17036. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"