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Product Name
ARHGDIB antibody
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Description
ARHGDIB Rabbit Polyclonal antibody. Positive IHC detected in human stomach cancer tissue. Positive WB detected in Jurkat cells, HL-60 cells, K-562 cells, Raji cells, rat spleen tissue. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 27 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARHGDIB antibody; GDIA2 antibody; GDID4 antibody; Ly GDI antibody; LYGDI antibody; RAP1GN1 antibody; Rho GDI 2 antibody; Rho GDI beta antibody; RhoGDI2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ARHGDIB recombinant protein (Accession Number: NM_001321423). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108180(ARHGDIB antibody) at dilution of 1:1000
IP Result of anti-ARHGDIB (IP:Catalog No:108180, 4ug; Detection:Catalog No:108180 1:1000) with Raji cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:108180(ARHGDIB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach cancer using Catalog No:108180(ARHGDIB antibody) at dilution of 1:100 (under 40x lens)
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Background
ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.
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References
- Fang Y, Yi J, Lizhi L, Qiucheng C. Rho GDP dissociation inhibitor beta promotes cell proliferation and invasion by modulating the AKT pathway in hepatocellular carcinoma. DNA and cell biology. 33(11):781-6. 2014.
- Zhang F, Liu H, Jiang G. Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line. Proteomics. 15(4):773-86. 2015.
- Geng J, Sun X, Wang P. Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity. Nature immunology. 16(11):1142-52. 2015.
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