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Product Name
Anti-Rab4 (2H11) Mouse antibody
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Description
Rab4 (2H11) Mouse monoclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Monkey
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human RAB4A expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
FC: 1/200 - 1/400
ELISA: 1/10000
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Validations
Western blot detection of Rab4 in Jurkat,A549,MCF7,C6 and Hela cell lysates using Rab4 mouse mAb (1:5000 diluted).Predicted band size:25KDa.Observed band size:25KDa.
Flow cytometric analysis of MCF-7 cells using RAB4A mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded liver cancer tissues using RAB4A mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using RAB4A mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P20338.The protein encoded by this gene belongs to the rab family. Rab4A protein is involved in the regulation of procathepsin L secretion, it was either inhibited by expression of a Rab4A dominant-negative mutant or increased by overexpression of the wild-type Rab4A in human melanoma cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"