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Product Name
Anti-POLR2E Rabbit antibody
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Description
POLR2E Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
RPB5; XAP4; RPABC1; hRPB25; hsRPB5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human POLR2E
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000
IHC: 1/20
FC: 1/20
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Validations
Western blot detection of POLR2E in K562,C6,3T3,Hela cell lysates using POLR2E Rabbit pAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.
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Background
Swiss-Prot Acc.P19388.DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"