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Product Name
Anti-MAP2 (1D7) Mouse antibody
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Description
MAP2 (1D7) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human, Mouse
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Alternative names
DKFZp686I2148;Dendrite specific MAP;DKFZp686I2148;MAP 2;MAP dendrite specific;MAP-2; MAP2;MAP2A;MAP2B;MAP2C;Microtubule associated protein 2;Microtubule-associated protein 2;Mtap 2;MTAP2_HUMAN. antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E.coli
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4 150mM NaCl)with 0.02% sodium azide 0.1%BSA 50%glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/150
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Validations
Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.
Western blot detection of MAP2(N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2(N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.
Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-MAP2(N-term) mouse mAb (dilution 1:100).
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Background
Swiss-Prot Acc.P11137.The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules.
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