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Product Name
Anti-GPM6B antibody
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Description
Rabbit polyclonal antibody to GPM6B
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Tested applications
WB, FC
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Species reactivity
Human, Mouse, Rat, Pig, Horse
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Alternative names
M6B antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human gpm6b 1-100/265
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1μg/Test
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Validations
Fig1: Sample:; U251(Human) Cell Lysate at 30 ug; Primary: Anti-GPM6B at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 29 kD; Observed band size: 34 kD
Fig2: Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.; Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 0.2μg in 100 μL1X PBS containing 0.5% BSA(green).
Fig3: Sample: Brain (Mouse) Lysate at 40 ug; Primary: Anti-GPM6B at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 29 kD; Observed band size: 36 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"