Anti-BACE1 antibody

Cat.#: 106525

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Product Information

  • Product Name
    Anti-BACE1 antibody
  • Documents
  • Description
    Rabbit monoclonal to BACE1
  • Tested applications
    FCM
  • Species reactivity
    Human BACE1 / ASP2
    Has cross-reactivity in ELISA with Mouse BACE1; Human ADAM15
  • Alternative names
    ASP2 antibody; BACE antibody; HSPC104 antibody; C76936 antibody; ASP2 antibody; BACE antibody; BACE1 antibody; Bace1 antibody; C76936 antibody; FLJ90568 antibody; HSPC104 antibody; KIAA1149 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human BACE1 / ASP2 (rh BACE1 / ASP2; NP_036236.1; Met 1-Thr 457) and conjugated with PE under optimum conditions, the unreacted PE was removed.
  • Clonality
    Monoclonal
  • Formulation
    Aqueous solution containing 0.5% BSA and 0.1% sodium azide
  • Storage instructions
    This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
    Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
  • Applications
    FCM
  • Validations

    BACE1 / ASP2 Antibody (PE), Rabbit MAb, Flow cytometric

    BACE1 / ASP2 Antibody (PE), Rabbit MAb, Flow cytometric

    Human BACE1 expression in Jurkat cells.The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and then stained with PE Rabbit anti-BACE1. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  • Background
    Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
  • References
    • Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.
    • Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.
    • Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.
    • Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"