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Product Name
ANP32B antibody
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Description
ANP32B Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, RAW 264.7 cells. Positive IP detected in HeLa cells. Positive IHC detected in human pancreas cancer tissue, human colon cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ANP32B antibody; APRIL antibody; PHAPI2 antibody; Silver stainable protein SSP29 antibody; SSP29 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANP32B recombinant protein (Accession Number: NM_006401). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108105(ANP32B antibody) at dilution of 1:1000
IP Result of anti-ANP32B (IP:Catalog No:108105, 3ug; Detection:Catalog No:108105 1:1000) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108105(ANP32B antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:108105(ANP32B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
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References
- Shen SM, Yu Y, Wu YL, Cheng JK, Wang LS, Chen GQ. Downregulation of ANP32B, a novel substrate of caspase-3, enhances caspase-3 activation and apoptosis induction in myeloid leukemic cells. Carcinogenesis. 31(3):419-26. 2010.
- Reilly PT, Afzal S, Wakeham A. Generation and characterization of the Anp32e-deficient mouse. PloS one. 5(10):e13597. 2010.
- Reilly PT, Afzal S, Gorrini C. Acidic nuclear phosphoprotein 32kDa (ANP32)B-deficient mouse reveals a hierarchy of ANP32 importance in mammalian development. Proceedings of the National Academy of Sciences of the United States of America. 108(25):10243-8. 2011.
- Yu Y, Shen SM, Zhang FF, Wu ZX, Han B, Wang LS. Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells. Biochemical and biophysical research communications. 423(4):721-5. 2012.
- Valdez BC, Zander AR, Song G. Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines. Blood cancer journal. 4:e171. 2014.
- Li CX, Shen SM, Wang LS, Yu Y. Caspase-3-resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis. Molecular medicine reports. 11(4):2813-8. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"